![]() Requires less hands-on training than tail or retro-orbital sampling to reliably withdraw a reasonable quantity of blood.Sample may be a mixture of venous and arterial blood.Repeated sampling is possible by alternating sides of the face.Obtainable blood volumes: medium to large.“Submandibular” or “Facial” vein/artery) Sampling: ** Maximum sample volume for that sampling frequencyīlood Collection methods in Mice and Rats (unanesthetized): Approximate blood sample volumes for a range of body weights are included in the table below: Body Weight (g) Volumes greater than recommended should be scientifically justified and appropriate fluid and/or cellular replacement provided. Similarly, of the circulating blood volume, approximately 10% of the total volume can be safely removed every 2 to 4 weeks, 7.5% every 7 days, and 1% every 24 hours. Marginal ear vein, cardiac (anesthetized, terminal) Saphenous, cardiac (anesthetized, terminal) ![]() Tail vein, saphenous vein, superficial temporal vein (a.k.a., "submandibular" or "facial"), cardiac (anesthetized, terminal), sublingual, jugularĬephalic, saphenous veins, femoral and jugular viens Superficial temporal vein (a.k.a., "submandibular" or "facial"), saphenous vein, tail vein, retro-orbital (anesthetized), cardiac (anesthetized, terminal) Note: If the amount of blood volume removed is 7.5% of total blood volume, allow 1 week recovery if amount removed is 10%, allow 2 weeks recovery if amount removed is 15%, allow 4 weeks recovery.Ĭommon Sites for Blood Collection: Species Source: Adapted from Formulary for Laboratory Animals, Hawk, Leary, and Morris 2005 Thus, 22.4 ml (10% of 224 ml) may be collected without giving replacement fluids once every two weeks.Įstimated Total Blood Volume and Safe Bleeding Volume of Selected Species: Species Non-terminal blood collection without additional monitoring (see below) should be limited to 10% of the total circulating blood volume on a single collection or every 2 week period for serial collections.Įxample (Using mean blood volume table below): a 4 kg rabbit is calculated to have a total blood volume of 224 ml (56 ml/kg x 4.0 kg). ![]() On average, the total circulating blood volume is equal to 5.5 -8.0 % of the animal’s body weight. Animals that are young, aged, stressed, have undergone experimental manipulations, or are suffering from cardiac or other disease conditions may not be able to tolerate these recommended blood volume withdrawals. The guidelines provided are for healthy, normal adult animals. Recommended volumes for blood collection are intended to preserve the health status of the animal and maintain the validity of experimental results. Please contact our Training Coordinator for details or to schedule a session. The amount of training and practice required to achieve a given level of competence in a particular technique varies from individual to individual depending on, for example, manual dexterity, prior experience, attitude, and the skills of the instructor.ĭLAR has a number of training resources available. Training and experience of the individual in the chosen procedure are of critical importance. Careful planning and control of blood sampling and all experimental variables associated with it should not only benefit the welfare of the animal, but also minimize confounding influences on research data. Researchers should plan and perform each sampling protocol with an appreciation for the stresses associated with blood loss to the animal and do whatever they can to minimize the animal's reaction to the stress. The method and volume of blood to be collected will depend on the animal species, frequency of collection, and experimental needs. ![]() The method of blood collection to be used, the intervals between blood collection procedures, and the volume of blood to be removed, should be listed in the approved protocol specific to each study. All procedures must be approved by the Institutional Animal Care and Use Committee (IACUC). This document is to provide guidance to investigators regarding safe blood collection volumes in common laboratory animals.
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